Cytochalasin B enhancement of the diacylglycerol response in formyl peptide-stimulated neutrophils.
نویسندگان
چکیده
منابع مشابه
Compartmentalization of cyclic GMP-dependent protein kinase in formyl-peptide stimulated neutrophils.
The presence and physiologic role of cyclic GMP-dependent protein kinase (G-kinase) in human neutrophils was investigated by Western blot analysis and immunocytochemistry. Small quantities of G-kinase were found in the cytoskeletal-enriched fraction of neutrophil lysates as detected by Western blots using a polyclonal antibody raised against bovine aorta G-kinase. Immunofluorescence microscopy ...
متن کاملRho GEF Lsc is required for normal polarization, migration, and adhesion of formyl-peptide-stimulated neutrophils.
Neutrophil migration requires continuous reorganization of the cytoskeleton and cellular adhesion apparatus. Chemoattractants initiate intracellular signals that direct this reorganization. The signaling pathways that link chemoattractant receptors to the cytoskeleton and cellular adhesion apparatus are now being defined. Formyl-peptide chemoattractants released from bacteria stimulate G-protei...
متن کاملDiacylglycerol accumulation and superoxide anion production in stimulated human neutrophils.
Exogenous diacylglycerols stimulate neutrophil superoxide anion production, suggesting that endogenous diacylglycerols may function as second messengers for this biological response. We have measured the diacylglycerol mass in human neutrophils stimulated by fMet-Leu-Phe, ionomycin, and concanavalin A and have correlated the kinetics and magnitude of the diacylglycerol response with those for s...
متن کاملSubcellular localization of H2O2 production in human neutrophils stimulated with particles and an effect of cytochalasin-B on the cells.
The ultrastructural localization of H2O2 production in suspended polymorphonuclear leukocytes (PMN) stimulated with particles was studied using CeCl3 technique. PMN stimulated with opsonized zymosan or polystylene latex with or without IgG were incubated in 0.1 M Tris-maleate buffer with 1 mM CeCl3 and 10 mM aminotriazole. Cells were then fixed and embedded in a resin for electron microscopy. T...
متن کاملSubcellular Localization of H202 Production in Human Neutrophils Stimulated With Particles and an Effect of Cytochalasin-B on the Cells
The ultrastructural localization of H202 production in suspended polymorphonuclear leukocytes (PMN) stimulated with particles was studied using CeCI3 technique. PMN stimulated with opsonized zymosan or polystylene latex with or without lgG were incubated in 0.1 M Tris-maleate buffer with 1 mM CeCI, and 10 mM aminotriazole. Cells were then fixed and embedded in a resin for electron microscopy. T...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)66649-9